Fsc1 localizes to the vacuole membrane and forms starvation-induced puncta.

(A) The predicted membrane topology and domain organization of Fsc1. (B) Localization of Fsc1 in non-starved cells. Cpy1 and Zhf1 are vacuole lumen and vacuole membrane markers, respectively. Bar, 3 µm. (C) Localization of Fsc1 in starved cells. Bar, 6 µm. (D) Time-lapse images of Fsc1 puncta induced by starvation. Bar, 3 µm. (E) Fsc1 puncta induced by starvation are dependent on Atg1, Atg11, and partially dependent on Atg13. Two hundred cells were examined for each data point.