Freshly isolated HBECs, and the H292 and BEAS-2B airway epithelial cell lines exhibit KCa3.1 channel activity.

Current-voltage curves demonstrating that 100 μM 1-EBIO induced characteristic KCa3.1 channel currents in freshly isolated HBECs isolated from (A) one patient with asthma (n = 3 cells) and (B) one healthy control (n = 3 cells). TRAM-34 (200 nM) blocked 1-EBIO-induced currents to near-baseline levels. Characteristic 1-EBIO-dependent KCa3.1 channel activity, sensitive to TRAM-34, was also seen in (C) the H292 cell line (n = 6 cells) and (D) the BEAS-2B cell line (n = 8 cells).