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Freshly isolated HBECs, and the H292 and BEAS-2B airway epithelial cell lines exhibit KCa3.1 channel activity.

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posted on 2015-12-23, 10:06 authored by Greer K. Arthur, S. Mark Duffy, Katy M. Roach, Rob A. Hirst, Aarti Shikotra, Erol A. Gaillard, Peter Bradding

Current-voltage curves demonstrating that 100 μM 1-EBIO induced characteristic KCa3.1 channel currents in freshly isolated HBECs isolated from (A) one patient with asthma (n = 3 cells) and (B) one healthy control (n = 3 cells). TRAM-34 (200 nM) blocked 1-EBIO-induced currents to near-baseline levels. Characteristic 1-EBIO-dependent KCa3.1 channel activity, sensitive to TRAM-34, was also seen in (C) the H292 cell line (n = 6 cells) and (D) the BEAS-2B cell line (n = 8 cells).

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    KCa 3.1 blockadeKCa 3.1 ion currentsKCa 3.1 blockersPrimary HBECsepithelial cell proliferationtgfepithelial fluid secretionKCa 3.1 mRNAHBEC functionKCa 3.1 currentsemtH 292 cellsairway epithelial biologySelective KCa 3.1 blockerstramHuman Bronchial Epithelial Cellsneoplastic H 292 epithelial cell lineKCa 3.1 protein expressionicaKCa 3.1 channelbeasKCa 3.1 K

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