Formation of eccDNA <i>in vitro</i> by cytosolic extract.

2013-02-21T05:28:07Z (GMT) by Zoya Cohen Sara Lavi
<p>Mouse genomic DNA was incubated with Hela cytosolic extract under conditions described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006126#s2" target="_blank">Materials and Methods</a> and the presence of 25 mM EGTA without additional supplies. The blot was hybridized to MSD.</p>