Food intake, caloric intake, change in body weight, and activity profiles of mice in experiment 2.

(A) Male mice fed high fat diet and cheese show a significant decrease in chow intake compared to mice with ad libitum access. (B) Male mice fed cheese and those fed high fat show a significant decrease in total caloric intake (sum of calories from chow plus and PM) in comparison to mice with ad libitum access. Caloric intake values were estimated from nutritional facts provided by the respective companies. (C) Male mice fed high fat keep a stable body weight, while mice fed cheese show a modest but statistically significant decrease in body weight after 21 days of PM access. AL controls show a gradual increase of body weight. Change in body weight is calculated by normalizing all body weights to the their respective value at day 0 (first day of PM presentation) (D) Fraction of time per hour spent walking, hanging, jumping, or rearing observed during each hour of the 24 hour recording of Day 14 of experiment 2. Mice fed high fat diet show statistically significant increase in high activity at ZT 9 (E) Fraction of time per hour spent sniffing food bin during 24-hour recording of Day 14. Both HF and CH groups show a statistically significant increase in food bin entry at ZT 8 (F) Fraction of time per hour spent hang cuddling during the 24-hour recording of Day 14. Mice fed high fat show an evident and statistically significant increase in hang cuddling at ZT 9. Arrows after ZT 9 below the y-axis indicate the time of PM presentation. For 1A thru 1C, bars represent means +/− SEM. Statistical comparisons were performed using an ANOVA followed by Tukey-Kramer multiple comparisons test. For 1D thru 1F, bars represent medians ± IQ Range (significance was tested with Mann-Whitney test with Dunn’s post test. * denotes p<0.05, ** denotes p<0.01.