Fluticasone propionate competes with phospho-GATA-3 for importin-α.

<p>(A) schematic representation of the in vitro binding competition assay. (B) GR isolated from FP (10<sup>−8</sup> M) stimulated cells enhances GR–importin-α binding in the presence (•) and absence (▪) of activated GATA-3. * <i>p</i><0.05 compared to no activated GR. (C) GATA-3 isolated from anti-CD3/CD28–stimulated cells does not attenuate GR–importin-α association. *<i>p</i><0.05 compared to control. (D) Activated GR blocks the ability of purified phospho-GATA-3 isolated from anti-CD3/CD28–stimulated cells interacting with immobilised importin-α in an in vitro binding assay. *<i>p</i><0.05 compared to GATA-3 isolated from unstimulated cells. <sup>#</sup><i>p</i><0.05 compared to stimulated GATA-3-importin binding. (E) The effect of activated (•) versus unstimulated (○) GR on attenuation of GATA-3–importin-α association was concentration-dependent. *<i>p</i><0.05, **<i>p</i><0.01 between groups. All results are expressed as mean±SEM of three independent experiments and analysed by ANOVA followed by Newman-Keuls post-test.</p>