Figure_2.tif (2.77 MB)
Flow-cytometric sort gates (A) and taxonomic composition (B) of single amplified genomes (SAGs) generated from coastal bacterioplankton using various fluorescent probes.
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posted on 2012-04-20, 00:10 authored by Manuel Martinez-Garcia, David M. Brazel, Brandon K. Swan, Carol Arnosti, Patrick S. G. Chain, Krista G. Reitenga, Gary Xie, Nicole J. Poulton, Monica Lluesma Gomez, Dashiell E. D. Masland, Brian Thompson, Wendy K. Bellows, Kai Ziervogel, Chien-Chi Lo, Sanaa Ahmed, Cheryl D. Gleasner, Chris J. Detter, Ramunas StepanauskasBacterioplankton were probed with (from top to bottom): 1) nucleic acid stain SYTO-9, targeting high- and low-nucleic acid content cells (HNA and LNA cells) representing a random subset of the entire microbial assemblage; 2) fluorescently-labeled laminarin; 3) fluorescently-labeled xylan; 4) 5-cyano-2,3-ditolyltetrazolium chloride (ETS-active cells) and 5) carboxyfluoresceindiacetate (esterase-active cells). Gates used for cell sorting are indicated in blue.
