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posted on 2014-04-17, 04:13 authored by Mary O′Connell Motherway, Debbie Watson, Francesca Bottacini, Tyson A. Clark, Richard J. Roberts, Jonas Korlach, Peggy Garault, Christian Chervaux, Johan E. T. van Hylckama Vlieg, Tamara Smokvina, Douwe van Sinderen

(A). Restriction analysis of total DNA from B. animalis subsp. lactis CNCM I-2494. Lane1, molecular weight marker X (Roche). Lane 2: Unrestricted total DNA from B. animalis subsp. lactis CNCM I-2494, Lane 3–5 total B. animalis subsp. lactis CNCM I-2494 DNA restricted with lane 3, HindIII; lane 4, AvaII and lane 5, EcoRI. (B) Restriction analysis of plasmid DNA isolated from three representative E. coli Xl1Blue -pWSK29-M.BanLII. Plasmid DNA from E. coli Xl1Blue -pWSK29-M.BanLII restricted with PstI and XbaI, lanes 2–4 (expected and obtained, product sizes of PstI +XbaI digests are 5420 bp and 1605 bp), or AvaII, lanes 5–7.

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