Figure 1

Figure 1A+B: Mitochondrial respiratory capacity in TA muscles from WT and KD mice treated with metformin or saline for two weeks. A: Mitochondrial respiration (O₂ flux) related to milligram muscle. B: Mitochondrial respiration (O₂ flux) related to milligram muscle and expressed relative to mitochondrial content (CS activity). Dark grey bars shows results in TA muscles from saline treated WT mice. Light grey bars shows results in TA muscles from saline treated KD mice. Dark grey bars with slashes shows results in TA muscles from metformin treated WT mice. Light grey bars with slashes shows results in TA muscles from metformin treated KD mice. ‡: Indicate significant difference between genotype within intervention (P<0.05). #: Indicate significant difference between interventions in AMPK KD mice (P<0.05). †: Indicate significant interaction between genotype and intervention (P<0.05). *: Indicate significant main effect of genotype (P<0.05). n = 11–14. Values are means ± S.E.M. Figure 1C: Substrate control ratio (SCR) is calculated as state 3 respiration with complex I and II linked substrates divided by state 3 respiration with complex I linked substrates. White bars shows results in TA muscles from saline treated mice. Black bars shows results in TA muscles from metformin treated mice. n = 11–14. Values are means ± S.E.M.