FRET analysis of subunit protein release triggered by ATP and GroES.

<p>Fluorescence intensities at wavelengths greater than 420<i>et al</i> <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078135#pone.0078135-Yoshimi1" target="_blank">[20]</a>. <i>Gray trace</i> indicates changes in FRET fluorescence in the presence of ATP only (no GroES), <i>green trace</i> indicates FRET changes in the presence of ATP and equimolar GroES. B. FRET traces for mutants. <i>Black trace</i> indicates SR-1, and <i>Gray trace</i> indicates D398A-RW. Note the differences in time scale between panels A and B.</p>