FH535 inhibits proliferation of LCSC and HCC cell lines.

<p>Cells were seeded in 96-well plates in 0.2 ml of media as described below for 72 hours, followed by the addition of <sup>3</sup>H-thymidine at 1 µCi/well for 4 hours. Incorporation of <sup>3</sup>H-thymidine was determined by scintillation counting. In panels A, B and D, the final concentration of DMSO in each well was 0.05%; in panel C, the final DMSO concentration in each well was 0.1%. (<b><i>A</i></b>) LCSCs were plated at 1000 cells/well in DMEM with 10% FBS along with DMSO alone or with increasing amounts of FH535. (<b><i>B</i></b>). LCSCs were plated at 5000 cells/well in DMEM with 1% FBS with DMSO alone or with increasing concentrations of FH535. (<b><i>C</i></b>). LCSCs were plated in DMEM with 10% FBS at 1000 cells/well with DMSO alone or increasing concentrations of XAV939. (<b><i>D</i></b>). Huh7, Hep3B and PLC cells were plated in DMEM with 10% FBS at 1000, 2500, and 5000 cells/well, respectively, with DMSO alone or increasing concentrations of FH535. <i>P</i> values are for all the three cell lines treated with FH535 are compared to controls. The experiment was done twice with similar results.</p>