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Expression of vTR harboring the mutant template (AU5) decreases cell proliferation of an avian cancer cell line.

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posted on 2013-02-20, 12:17 authored by Benedikt B. Kaufer, Sina Arndt, Sascha Trapp, Nikolaus Osterrieder, Keith W. Jarosinski

A) Analysis of telomerase activity in primary CEC cultures, the chicken fibroblast cell line DF-1 with or without TERT expression, and the quail QT35 cancer cell line using TRAP assays. TRAP products representing telomere elongation and internal control (IC) are indicated. B) RT-qPCR of vTR copies in polyclonal empty vector, vTR or AU5 QT35 cell lines induced with 1 µg/ml doxycycline (Dox) for 3 and 5 d or left uninduced. Data is shown as relative quantitation (RQ) of vTR copies relative to quail GAPDH RNA copies that served as an endogenous control. C) Percent (%) confluency of vector, vTR, and AU5 cell lines over the course of 31 d. Results are shown as means and standard errors of three independent experiments. P values were determined between each group using Student's t tests.

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