Expression of genes that were changed in both 3 and 10% cigarette smoke extract by 1.5 fold or above compared to control in PBMCs.

<p>PBMCs were treated for 8 hours or 24 hours with RPMI-1640 medium (control 1–3), 3% CSE (1–3) or 10% CSE (1–3). RNA was extracted from each sample and gene expression values measured using the Illumina HumanRef-8v3 BeadChip array. (<b>A</b>) Heat map representing normalized signal intensity values and list of genes altered by ≥1.5 fold in PBMCs treated with both 3 and 10% CSE after 8 hours. Red denotes high expression and turquoise denotes low expression. Order of samples was dictated by hierarchical clustering. (<b>B</b>) Heat map representing normalized signal intensity values and list of genes altered by ≥1.5 fold in PBMCs treated with both 3 and 10% CSE after 24 hours. Red denotes high expression and turquoise denotes low expression. Order of samples was dictated by hierarchical clustering. Statistical significance (p<0.05) was calculated using one-way analysis of variants followed by a Tukey's post-hoc test and Benjamini-Hochberg FDR correction on GeneSpring GX11.0.2 software. Fold change represents a comparison between mean normalised signal intensity for control (n = 3) versus smoke (n = 3) treated PBMCs. Refer to Data S2 for full data sets and Entrez Gene IDs.</p>