Expression of genes that were changed in both 3 and 10% cigarette smoke extract by 1.5 fold or above compared to control in PBMCs.

PBMCs were treated for 8 hours or 24 hours with RPMI-1640 medium (control 1–3), 3% CSE (1–3) or 10% CSE (1–3). RNA was extracted from each sample and gene expression values measured using the Illumina HumanRef-8v3 BeadChip array. (A) Heat map representing normalized signal intensity values and list of genes altered by ≥1.5 fold in PBMCs treated with both 3 and 10% CSE after 8 hours. Red denotes high expression and turquoise denotes low expression. Order of samples was dictated by hierarchical clustering. (B) Heat map representing normalized signal intensity values and list of genes altered by ≥1.5 fold in PBMCs treated with both 3 and 10% CSE after 24 hours. Red denotes high expression and turquoise denotes low expression. Order of samples was dictated by hierarchical clustering. Statistical significance (p<0.05) was calculated using one-way analysis of variants followed by a Tukey's post-hoc test and Benjamini-Hochberg FDR correction on GeneSpring GX11.0.2 software. Fold change represents a comparison between mean normalised signal intensity for control (n = 3) versus smoke (n = 3) treated PBMCs. Refer to Data S2 for full data sets and Entrez Gene IDs.