Expression of cholinergic receptor muscarin 3 and acetylcholine esterase in eccrine gland secretory coil of normal and atopic dermatitis skin specimens.
(A) Representative immunohistochemical staining of cholinergic receptor muscarin 3 (CHRM3) and acetylcholine esterase (AchE). Bars indicate 50 μm. (B) The staining intensities are expressed as RD. The RD of CHRM3, but not AchE, is significantly lower in AD patients. Results are expressed as mean ± SEM. (C) Double immunofluorescence staining of CHRM3 and GCDFP15 in the eccrine gland secretory coil. In eccrine gland secretory coil cells, the expression levels of CHRM3 and GCDFP15 are decreased in parallel in AD. Bars indicate 50 μm.