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Expression of Tim-3 on TILs in mouse model of transplantable tumor.

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posted on 2012-02-17, 00:18 authored by Xin Gao, Yibei Zhu, Gang Li, Haitao Huang, Guangbo Zhang, Fengming Wang, Jing Sun, Qianting Yang, Xueguang Zhang, Binfeng Lu

6–8-week-old C57BL/6 mice were inoculated with 2×105 B16F0 cells i.d., tumor samples, spleens, and lymph nodes were removed when tumor sizes reached around 15 mm in diameters on day 20. TILs, splenocytes, and lymph node cells were isolated for analysis by flow cytometry. Tim-3 expression on mouse CD4+Foxp3+ or CD4+Foxp3 T cells is shown. Data shown are representative of five independent experiments.

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