Expression of Tim-3 on TILs in mouse model of transplantable tumor.

<p>6–8-week-old C57BL/6 mice were inoculated with 2×10<sup>5</sup> B16F0 cells i.d., tumor samples, spleens, and lymph nodes were removed when tumor sizes reached around 15 mm in diameters on day 20. TILs, splenocytes, and lymph node cells were isolated for analysis by flow cytometry. Tim-3 expression on mouse CD4<sup>+</sup>Foxp3<sup>+</sup> or CD4<sup>+</sup>Foxp3<sup>−</sup> T cells is shown. Data shown are representative of five independent experiments.</p>