Expression of <i>Ret</i> and its signalling partners in adult thymic populations.

<p><b>A.</b> DN, DP, SPCD8<sup>+</sup> and SPCD4<sup>+</sup> thymocytes were purified by flow cytometry. Results show quantitative RT-PCR normalized to <i>Hprt1</i>. Error bars show s.e.. Results from three independent measurements are represented. <b>B.</b> RET expression in DN and DP thymocytes was determined by flow cytometry. RET: black bold line; Isotype control: grey line. <b>C.</b> Thymic DN, DP, SPCD8<sup>+</sup> and SPCD4<sup>+</sup> thymocytes and CD45<sup>−</sup> cells were purified by flow cytometry. Quantitative RT-PCR analysis was normalized to <i>Hprt1</i>. Error bars show s.e.. Results from three independent measurements are represented. <b>D.</b> DN1–2 and DN3–4 thymocytes were purified by flow cytometry. Results show quantitative RT-PCR normalized to <i>Hprt1</i>. Error bars show s.e.. Results from three independent measurements are represented.</p>