Expression of PxylOR1 and PxylOR4 genes in male antenna of P. xylostella.

In situ hybridizations were performed with digoxigenin-labelled antisense RNA probes on longitudinal tissue sections of male antennae. Signals were visualized using an anti-DIG antibody. (A) Hybridization signals in one segment of the P. xylostella antenna are shown. (B) Negative control with a DIG-labeled sense probe. (C) Higher magnification of long trichoid sensilla with hybridization signals. (D) to (G) No hybridization signal was detected under short trichoid sensilla, basiconi sensilla, coeloconic sensilla and chaetica sensilla. Scale bars: 5 µm in A–B and 2 µm in C–G.