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Expression of PLDs in response to various stresses.

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posted on 2013-02-20, 10:59 authored by Jian Zhao, Cunxi Wang, Mohamed Bedair, Ruth Welti, Lloyd W. Sumner, Ivan Baxter, Xuemin Wang

(A) RNA blotting of PLDα1 and PLDγ transcripts in Arabidopsis leaves exposed to different stress treatments. Arabidopsis Col-0 (3–week old) leaves were detached and floated on water without or with indicated concentrations of chemicals, 1% NaCl, 0.6 M mannitol, 0.2 mM AlCl3, 0.2 mM CdCl2, 2 mM H2O2, 0.1 mM salicylic acid (SA), 0.1 mM methyl salicylate (MeSA), 0.1 mM abscisic acid (ABA), or 0.1 mM methyl jasmonate (MeJA) in a growth chamber at 22°C. Leaves were collected at the indicated times for RNA extraction and northern blotting with [α-32P]-labeled PLDα1 or PLDγ1 full-length cDNA as a probe. (B) RNA blotting of PLDγs using the coding region of PLDγ1 (upper panel) or PLDγ2-specific 5′-UTR (lower panel) under Al stress. Two week-old wild-type seedlings grown in ½ MS medium were treated with 100 µM AlCl3, and roots of seedlings were collected at the indicated time for RNA extraction. [α-32P]-Labeled PLDγ1 cDNA or PLDγ2- specific 5′-UTR was used as a probe for northern blotting. EtBr-stained ribosomal RNA was used as a loading control.

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