Expression of Met, gce and Kr-h1 is reduced in the Axn, slmb and nkd mutants.

(A) Total RNAs were extracted from Oregon R, Axn^EY10228, slmb⁰⁰²⁹⁵, and nkd² 2^nd instar larvae. The mRNA levels of Met, gce and Kr-h1 were assessed by quantitative real-time PCR and normalized to rp49 mRNA. Values shown are the means of 4 independent experiments ± standard deviations. (B) The same total RNAs described in [A] were used as the templates for a 30-cycle reverse transcriptional PCR. The RT-PCR products were analyzed by DNA agarose gel electrophoresis.