Expression analysis of selected DevR regulon transcripts.
Various M. tb Comp strains were grown to an A595 of 0.2–0.3 under aerobic conditions and kept standing for 5 days (hypoxic cultures). Gene expression was assessed in Comp strains generated in Mut1* and Mut2ΔdevR backgrounds by qRT Reverse transcriptase-PCR analysis. Fold change in target gene expression in hypoxic vs. aerobic cultures was calculated from normalized transcript levels with respect to 16S rRNA. Mean fold change ± SD determined from three independent cultures is shown.