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Ylr456w and Ypr172w proteins don’t bind FMN.

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posted on 2015-09-01, 03:06 authored by Tatiana Domitrovic, Diana P. Raymundo, Tiago Fernandes da Silva, Fernando L. Palhano

FMN fluorescence (1 μM) under Ylr456w, Ypr172w and PDX3 titration after incubation at 25°C for 2 min in buffer A in the presence of 100 μM PNP (A) or 100 μM PLP (B). Ex = 450 nm, Em = 520 nm. The error bars represent the standard deviation of two independent experiments with two different proteins batches. Statistical test used was 2way ANOVA Dunnett’s multiple comparison test where *, p<0.05; **, p<0.01; ***, p<0.001 and ****, p<0.0001.

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