Exercise preserves neurovascular unit integrity in aged mice.

<p>(A–B) Age-related intravascular and extravascular deposition of FIBRIN (red) is significantly reduced in aged runner mice. Microvessels are immunostained with endothelial CD31 (cyan). (C) PDGFRβ<sup>+</sup> pericyte (magenta) number and coverage are significantly increased in aged runners when compared with aged sedentary mice. (D) Quantitative analysis of PDGFRβ<sup>+</sup> pericytes number and coverage. (E) AQP4 (blue) immunoreactivity of astrocyte endfeet is significantly increased in aged runners when compared with aged sedentary mice. (F) AQP4 protein levels show a significant preservation of both parameters on aged runner mice. In panels (D–F), values are mean <u>+</u> SEM, <i>n</i> = 4 aging mice, <i>n</i> = 6 aged Sd mice and <i>n</i> = 6 aged Rn mice. In (B) *<i>p</i> = 0.014 and (F) *<i>p</i> = 0.0318 by unpaired <i>t</i> test. In (D) ****<i>p</i> < 0.0001 and ***<i>p</i> = 0.0014 by ANOVA followed by Tukey’s posthoc tests. Scale Bars: 50 μm. The data used to make this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002279#pbio.1002279.s001" target="_blank">S1 Dataset</a>.</p>