Evolutionary Conserved Lipid Droplet Regulators

Mouse AML12 cells store little lipid in the absence of exogenous NEFA (A), whereas addition of NEFA to the growth medium induces lipid droplet deposition (B). Knockdown effects on cellular lipid droplets of the mouse genes encoding Uqcrq (CG7580 in Drosophila), an electron transport chain member, and Smarca4 (Brahma in Drosophila), a chromatin-associated protein (C and D). Nuclei are shown in blue (stained with Hoechst 33342), lipid droplets in green (stained with BODIPY493/503). Scale bar in (A) represents 50 μm.