Evidence at the protein level for nesprin-2-epsilon-1 in Ntera-2 and ESC cells and for nesprin-2-epsilon-2 in heart and brain.

<p>Western blot for nesprin-2 in human tissues and cell lines using antibody against the C-terminal region of nesprin-2 (MANNES2A 11A3). Bands of approximate size of nesprin-2-epsilon-1 (122 kD) were detected in Ntera-2 and ESC and bands the size of nesprin-2-epsilon-2 (103 kD) were detected in brain and heart. Nesprin-2-alpha-1 was observed in skeletal muscle, but epsilon isoforms were not detected. Absence of mRNA (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094380#pone.0094380.s001" target="_blank">Table S4 in File S1</a>), indicates that putative nesprin-2-gamma (377 kD) and nesprin-2-alpha-2 (47 kD) bands on the skeletal muscle western blot, are likely to be degradation products.</p>