Evidence at the protein level for nesprin-1-beta-1 in spleen and for nesprin-1-alpha-2 in cardiac and skeletal muscle.

Western blot for nesprin-1 in tissues using antibody against the C-terminal region of nesprin-1 (MANNES1E 8C3). A band the size of nesprin-1-beta-1 (383 kD) was detected in spleen and bands of nesprin-1-alpha-2 (111 kD) were detected in skeletal muscle and heart.