Evaluation of the transposition efficiency mediated by the gradient concentrations of PBase.

(A) The NP-mPB group demonstrated more colonies compared to the mPB group in HEK293T cells transfected with varying amounts of plasmid DNA. (B) Colony numbers were quantified, revealing a 2.5- to 6-fold increase in transposon integration efficiency for NP-mPB. The transposition efficiency was higher when more plasmid was transfected for both groups. No overproduction inhibition was noted. n  = 3 for each condition; bars indicate mean ± standard error; ** P<0.01, *** P<0.001.