Evaluation of shRNA transfection-efficiency and integrin β3 deficiency.

<p><b>A)</b> Fluorescence pictures of cells with shRNAs transiently transfected for 24 h. 976, 1110 and 1755 denote the targeting positions on integrin β3 mRNA sequence. nc denotes the negative-control construct which generates non-specific interfere RNA. <b>B)</b> Fluorescence pictures of cells transiently transfected with shRNA976, shRNA1110, shRNA1755 or nc for 48 h. <b>C)</b> Quantitative analysis of fluorescence intensity in Fig. 6A and 6B evaluated by ImageJ. <b>D)</b> Assessment of integrin β3 deficiency at 48 h post shRNA transiently transfection using two-step qPCR. ***p<0.001 denotes extremely significant difference. n.s. denotes no significant difference.</p>