Etk and Wzc are not essential for Etp phosphorylation.

EtpD119A was expressed in different EPEC strains with deletions in the etp gene and the kinase genes etk and wzc. Proteins were extracted and the amount and phosphorylation state of Etp were tested by Western blot using anti-6His and anti-PY antibodies, respectively. The presence of intact genes is indicated above the lanes.

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