Eps15-KO mice show increased marginal zone B cell numbers.

<p><b>A.</b> Dot plots of bone marrow cells from 4-month-old Eps15-WT and Eps15-KO mice stained for B220 and IgM to identify pre−/pro-B cells (B220<sup>+</sup>IgM<sup>−</sup>), immature (B220<sup>+</sup>IgM<sup>+</sup>) and mature (B220<sup>high</sup>IgM<sup>+</sup>) B cells. Pre−/pro-B cells were further analyzed for expression of CD43 to identify pro- (CD43<sup>+</sup>) and pre- (CD43<sup>−</sup>) B cells. <b>B.–C.</b> Dot plots depicting the total number of mature (B220<sup>++</sup>), immature (Imm.) and pre−/pro- and B cells (<b>B</b>) and pre- and pro-B cells (<b>C</b>) in the bone marrow of Eps15-WT (WT, white symbols, n = 5) and Eps15-KO (KO, black symbols, n = 6) mice. <b>D.</b> Top panel: dot plot of thymocytes from 2-month Eps15-WT and Eps15-KO mice stained for CD19 and TCRβ to identify B and T cells, respectively. Middle panel: dot plots for thymocytes from 4-month-old Eps15-WT and Eps15-KO mice staind for CD4 and CD8 to identify thymocyte subpopulations. Bottom panel: the CD4/CD8 double negative population (DN) was further analyzed for immature thymocytes using CD44 and CD25. <b>E.–G.</b> Dot plots depicting the total number of the following cell populations in the thymi of Eps15-WT (WT, white symbols) and Eps15-KO (KO, black symbols) mice: (<b>E</b>) TCRβ<sup>+</sup> and CD19<sup>+</sup> cells (n = 3), (<b>F</b>) DN (double negative, CD4<sup>−</sup>CD8<sup>−</sup>), DP (double positive, CD4<sup>+</sup>CD8<sup>+</sup>), CD4<sup>+</sup> and CD8<sup>+</sup> (n = 4) and (<b>G</b>) of DN1 (CD44<sup>+</sup>CD25<sup>−</sup>), DN2 (CD44<sup>+</sup>CD25<sup>+</sup>), DN3 (CD44<sup>−</sup>CD25<sup>−</sup>) cells (n = 4). <b>H</b>. Dot plot of peritoneal B cells from 2–4 month old Eps15-WT and Eps15-KO mice stained for CD19 and B220 gated for CD19<sup>+</sup>B220<sup>low</sup> B1 and CD19<sup>+</sup>B220<sup>high</sup> B2 B cells. B1 B cells were further stained and gated for CD5 to identify CD5<sup>+</sup> B1a and CD5<sup>−</sup> B1b B cells. <b>I.–J.</b> Dot plots depicting the percentage of B1 and B2 (I) and B1a and B1b (J) B cells in the peritoneum of Eps15-WT (WT, white symbols) and Eps15-KO (KO, black symbols) mice (n = 8). <b>K.</b> Dot plot of splenocytes from Eps15-WT and Eps15-KO mice stained for CD19 and gated for CD21 and CD23 to identify Fo B (CD19<sup>+</sup>/CD21<sup>+</sup>/CD23<sup>+</sup>) and MZ B (CD19<sup>+</sup>/CD21<sup>+</sup>/CD23<sup>−</sup>) cells. <b>L.</b> Dot plots depicting the total number of CD19+, Fo B and MZ B cells in the spleens of Eps15-WT (WT, white symbols) and Eps15-KO (KO, black symbols) mice (n = 5). Values are depicted as mean±standard error mean. Statistical significance was assessed using Student’s t-test and significant differences are indicated as * = p<0.05.</p>