Enzymatic activity assay in vitro with serial amount of enzyme at 27 °C or 37 °C.
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0.25-5 μg bacterially expressed Spodoptera His-Su(var) 3-9 (~70 kDa) was added in the reaction, the “5+” indicates the presence of 5 µM Chaetocin in the reactions containing 5 µg recombinant His-Su(var) 3-9. Reaction product was analyzed with Western blot and the bands of trimethylated H3K9 and total H3 (~18 kDa) were shown in the upper and middle panel, respectively. The SDS-PAGE of reaction mixtures was shown at bottom panel.