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Enhanced basal synaptic transmission and LTP in hippocampal slices from mice pre-treated with SK2 antisense ODNs.

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posted on 2015-05-04, 03:29 authored by Saravana R. K. Murthy, Tessi Sherrin, Chad Jansen, Ingrid Nijholt, Michael Robles, Amalia M. Dolga, Nicolas Andreotti, Jean-Marc Sabatier, Hans-Guenther Knaus, Reinhold Penner, Cedomir Todorovic, Thomas Blank

The distance between the stimulating and recording electrodes was kept constant between slices. (A) Input-output curve of fEPSP slope (mV/ms) versus stimulus (V) at the SC-CA1 pyramidal cell synapse in naive mice and mice pre-treated with vehicle, antisense ODNs against SK2 channels and control ODNs. The maximal fEPSP slopes were significantly larger in the SK2 antisense-treated mice than those in the naive, vehicle and control ODNs-treated mice. Data are presented as mean ± s.e.m. (B) Relationship between the slope of the evoked fEPSPs from panel A and the corresponding fiber volley amplitude. SK2 antisense-treated mice exhibit a greater postsynaptic response than control groups to similar presynaptic depolarization. Data are presented as mean ± s.e.m. (C) Comparison of PPF in naive mice and mice pre-treated with vehicle, antisense ODNs against SK2 channels and control ODNs. No differences were found between these four groups of mice. Data presented are the mean ± SEM of the facilitation of the second response relative to the first response. Insets: Field EPSPs recorded in response to paired-pulse stimulation at an interstimulus interval of 50 ms in slices from all four treatment groups as indicated. (D) TBS-LTP elicited in slices from mice that were pre-treated with SK2 antisense ODNs was significantly enhanced when compared to LTP induced in slices from naive mice. There was no statistical difference between control ODNs-injected mice and mice that were pre-injected with vehicle. Insets: Responses shown are fEPSPs recorded during baseline (upper row) and 55–60 min (bottom row) after the induction of LTP (post-induction). Traces are averages of five consecutive responses. Statistical significance was determined by two-way ANOVA followed by Bonferroni multiple comparisons test (*p < 0.05).

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