Electrophoretic result of PCR products and multiple alignment of amino acid sequences.

(A) The PCR product for H3. M, DM2000 DNA marker; 1, PCR product of E. sinensis H3. (B) Multiple alignment of deduced H3 amino acid sequence in E. sinensis with those of other species: Homo sapiens, Mus musculus, Drosophila simulans, Danio rerio, Xenopus laevis, Saccharomyces cerevisiae. (C) The PCR product for H4. M, DM2000 DNA marker; 1, PCR product of E. sinensis H4. (D) Comparison of deduced amino acid sequence of E. sinensis histone H4 with other peptides. Symbol key: *the same amino acid; “:” amino acid sequence with strong similar property; “.” amino acid sequence with weak similar property.