Electrophoretic analyses of Lcc4A, Lcc5, and Lcc7.

(A) SDS-PAGE of recombinant laccases. Proteins were denatured before being resolved in 12% (w/v) SDS-PAGE followed by CBBR staining. (B) Zymogram of recombinant laccases. Native proteins were resolved in 12% (w/v) SDS-PAGE followed by an activity staining with 1 mM ABTS in accordance with Srinivasan et al. [23].