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Efficient invalidation of TMEM45A in RHE after 11 days of reconstruction (A-C) and in monolayer culture of human keratinocytes (D-F).

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posted on 2016-01-28, 12:37 authored by Aurélie Hayez, Edith Roegiers, Jérémy Malaisse, Benoit Balau, Christiane Sterpin, Younes Achouri, Catherine Lambert De Rouvroit, Yves Poumay, Carine Michiels, Olivier De Backer

(A) Relative quantification of TMEM45A mRNA levels in epidermis reconstructed with keratinocytes transduced with NT shRNA or shRNA targeting TMEM45A. Bars represent 95% confidence intervals. Paired t-test (n = 3, **p≤0.1). (B) Morphology of HE-stained epidermis. Bars: 50 μm. (C) Immunofluorescence detection of TMEM45A in RHE. Bars: 50 μm. (D) Relative mRNA quantification in monolayers at subconfluence (SC), confluence (C) and post-confluence (PC). Bars represent 95% confidence intervals. ANOVA 2 (n = 3, *** p≤0.001). (E) TMEM45A abundance analysis by WB in post-confluent monolayer culture. RPL13a is the loading control. (F) Detection of TMEM45A in confluent monolayers culture. Bars: 25 μm. (B, C, E, F). The presented data are representative of the results obtained for at least three independent experiments.