Effects of uniqueness normalization on expression level.

<p>(<b>A</b>) Histogram showing how uniqueness compensation using MULTo affects the RPKM values at different read lengths. The x-axis show the difference in gene expression between uniqueness compensated and uncompensated expression levels. (<b>B</b>) RPKM values for FTH1 before and after uniqueness normalization. (<b>C</b>) Read coverage and uniqueness profile across FTH1 for 25 nt reads. Uniqueness density was calculated as the proportion unique reads aligning to each genomic coordinate.</p>