Effects of <i>Tcl1</i> deficiency and overexpression on ES-cell differentiation.

<p>The expression of representative stem and differentiation markers was examined by real time PCR in ES cells grown in LIF(+) culture (left half of each panel) and with EB formation (right half of each panel). For EB formation, trypsinized ES cells were seeded into a bacterial grade dish, and cultured for 12 days. Values are expressed as mean ± SEM of three technical replicates. ∗<i>P</i><0.05 and ∗∗<i>P</i><0.01 by Student’s t-test. <i>Tcl1<sup>−/−</sup></i> (KO) vs. wild-type (WT) or <i>Tcl1<sup>−/−</sup></i>(CAG-<i>Tcl1</i>) cells.</p>