Effects of RNAi-mediated silencing of methyl farnesoate epoxidase (<i>mfe</i>) gene function on major control genes of JH biosynthesis and metabolism and on the hemolymph JH titer.

<p>Newly-emerged adult workers were injected with 3 µg <i>mfe</i> dsRNA or GFP dsRNA or left untreated. The bees were collected 7 days after the treatment. (A) <i>mfe</i> transcript levels determined by RT-qPCR using whole body RNA. (B) JH hemolymph titers measured by RIA. (C) <i>jhe</i> transcript levels determined by RT-qPCR using whole body RNA. <i>rp49</i> was used for normalization. Each bar represents the mean ± SEM of independent samples; sample size is shown above bars. Different letters represent statistical differences assessed by One-way ANOVA followed by Tukey's <i>post hoc</i> test; (p≤0.001 in A and C; p = 0.003 in B).</p>