Effective reduction of DRBD3 expression by RNAi based on p3666.

<p>(A) Diagram of p3666, the integration into the genome after NotI digestion is the same as shown for p3227 in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035167#pone-0035167-g002" target="_blank">Fig. 2A</a>. The arrangement of the restriction sites within the stem multiple cloning sites (MCS) is shown. (B) Growth of cells expressing DRBD3-eYFP with or without tetracycline addition. Experiment was repeated with three clones and a representative growth curve is shown. Red line is with tet induction; blue is without tet induction. (C) Western blot probed with anti-DRBD3 of samples collected over a time course after induction of RNAi. The levels of DRBD3 and DRBD3-eYFP expression were normalised against the BiP loading control. Experiment was repeated with three clones and a representative Western blot and quantitation is shown.</p>

Keyword(s)

License

CC BY 4.0