Effective reduction of DRBD3 expression by RNAi based on p3666.
(A) Diagram of p3666, the integration into the genome after NotI digestion is the same as shown for p3227 in Fig. 2A. The arrangement of the restriction sites within the stem multiple cloning sites (MCS) is shown. (B) Growth of cells expressing DRBD3-eYFP with or without tetracycline addition. Experiment was repeated with three clones and a representative growth curve is shown. Red line is with tet induction; blue is without tet induction. (C) Western blot probed with anti-DRBD3 of samples collected over a time course after induction of RNAi. The levels of DRBD3 and DRBD3-eYFP expression were normalised against the BiP loading control. Experiment was repeated with three clones and a representative Western blot and quantitation is shown.