Effect of venoms on human keratinocytes viability.
2013-08-22T02:14:04Z (GMT) by
<p>HaCaT cells (5×10<sup>4</sup> cells/well) were cultured in 96-well plates with DMEM without FBS for 24 hours followed by incubation with venoms (10 µg of protein). After 48 h and 72 h, the viability was tested by the MTT method and the readings taken at wavelengths of 540–620 nm. Data are expressed as mean ± SD of duplicates. (*) Significant differences (<i>P</i><0.05) from control; (#) Significant differences from <i>L. laeta</i> venom (<i>P</i><0.05); (•) Significant difference between female and male <i>Sicarius ornatus</i> venoms (<i>P</i><0.05).</p>