Effect of resveratrol and/or metformin on SIRT1-mediated signaling and senescent “memory” in HUVECs.

Immunoblotting and quantification of SIRT1 protein expression after (A-B) resveratrol treatment (RSV; 0, 1.25, 2.5, 5, or 10 μM) or (C-D) metformin treatment (MET; 0, 1, 10, 50, 100, 250 μM). Values were normalized to β-actin. (E-J) Immunoblotting and quantification of p300, SIRT1, p21, Ac-p53, and p53 protein expression after RSV (5 μM) or MET (50 μM) treatment. For p300, SIRT1, and p21, values were normalized to β-actin; for Ac-p53, normalized to total p53. (K) The deacetylase activity of SIRT1 in HUVECs treated with or without RSV (5 μM) or MET (50 μM). (L) The percentage of SA β-Gal positive cells in HUVECs treated with or without RSV (5 μM) or MET (50 μM). *P < 0.05.