Effect of rCD93 domain proteins on the chemotactic migration and MMP secretion of HUVECs.
2012-12-18T00:54:42Z (GMT) by
<p>A migration assay was performed in Transwell. (A) HUVECs (1 × 10<sup>5</sup> cells) suspended in M199 supplemented with 1% FBS were added to the upper wells. The lower wells were filled with different concentrations of rCD93D123 (A) or rCD93D23 (B), or with 10 ng/ml of VEGF. (C) The lower wells were filled with rCD93D23 (30 ng/ml) in the absence or presence of CD93D23 Ab (5 µg/ml), boiled rCD93D23, or 10 ng/ml of VEGF. Each value represents the mean ± SD (n = 3), and similar results were obtained in three independent experiments. *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001 vs. medium alone. ###, <i>p</i><0.001 vs. rCD93D23. (D) The culture media of HUVECs treated with different concentrations of rCD93D23 or VEGF (10 ng/ml) were used in gelatin zymography. The similar results were obtained in three independent experiments. ***, <i>p</i><0.001 vs. medium alone.</p>