Figure_4.tif (4.63 MB)

Effect of mutations on expression of the 208 core root epidermal genes.

figure

posted on 2012-01-12, 00:51 authored by Angela Bruex, Raghunandan M. Kainkaryam, Yana Wieckowski, Yeon Hee Kang, Christine Bernhardt, Yang Xia, Xiaohua Zheng, Jean Y. Wang, Myeong Min Lee, Philip Benfey, Peter J. Woolf, John Schiefelbein

(A) Low magnification view of roots from wild-type and homozygous root epidermis mutants. Scale bar: 250 µm. (B) Hierarchical clustering of the 208 core root epidermal genes, based on their relative transcript accumulation in Affymetrix ATH1 microarrays using WER::GFP-expressing cells from three replicates of (left to right) the cpc try, rhd6, gl2, ttg, gl3 egl3, wer myb23 mutants and the wild type Columbia. Red = high transcript level; Blue = low transcript level. Asterisks indicate genes significantly affected in the rhd6 mutant background. (C) Hierarchical clustering of the 208 core root epidermal genes, based on their relative transcript accumulation (averaged from three replicates) in Affymetrix ATH1 microarrays using WER::GFP-expressing cells from (left to right) cpc try, rhd6, csld3, mrh3, gl2, gl3 egl3, ttg, lrx1, cobl9, rhd2, ire, mrh1, mrh2, wild type, cow1, myc1, bhlh66, and wer myb23. Red = high transcript level; Blue = low transcript level. Heirarchical clustering of the complete set of three replicates for each line is presented as Figure S5.