Effect of miR-19a target genes on lung cancer cells.

<p>(A) Relative cell viability of A549 cells 24 h after transfection of expression plasmids with miR-19a target gene cDNAs. (B) Relative cell viability of LK79 cells 24 h after transfection of expression plasmids with miR-19a target gene cDNAs. (C) Colony formation of A549 cells (<i>upper</i>) and LK79 cells <i>(lower)</i> 3 weeks after transfection with miR-19a target cDNA plasmids and G418 selection. Each assay was performed in 3 wells of 6-well plates, and the representative images of the results are shown. (D) Average colony number of A549 cells (<i>left</i>) and LK79 cells <i>(right)</i> in the colony formation assay. **, <i>p</i> < 0.005 using a two-tailed <i>t</i>-test.</p>