Effect of miR-19a target genes on lung cancer cells.

(A) Relative cell viability of A549 cells 24 h after transfection of expression plasmids with miR-19a target gene cDNAs. (B) Relative cell viability of LK79 cells 24 h after transfection of expression plasmids with miR-19a target gene cDNAs. (C) Colony formation of A549 cells (upper) and LK79 cells (lower) 3 weeks after transfection with miR-19a target cDNA plasmids and G418 selection. Each assay was performed in 3 wells of 6-well plates, and the representative images of the results are shown. (D) Average colony number of A549 cells (left) and LK79 cells (right) in the colony formation assay. **, p < 0.005 using a two-tailed t-test.