Effect of gAd on H/R-induced DNA fragmentation detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using laser scanning cytometry and phosphatidylserine externalization detected by Annexin V labeling.
Shown in (A) is a representative scatterplot for each condition (normoxia, H/R: 21 h hypoxia followed by 6 h reoxygenation, H/R+gAd (2.5 µg/ml)) where above the horizontal line indicates TUNEL positive cells (red). The quantitative analysis of % TUNEL positive cells is shown inset. Values shown are mean ± SEM from three separate analyses. (B) shows representative immunofluorescent confocal images and the quantitative analysis of intensity of Annexin V positive fluorescence. Values shown are mean ± SEM from n = 3. * indicates p<0.05 with respect to normoxia and # indicates p<0.05 with respect to H/R.