Effect of extracellularacyl-CARs on IhERG.
IhERG-V relationships,in all graphs, the filled squares represent the current in the absence of acyl-CAR in the pipette (control, n = 12 cells). Aa, effect of 3 µM C8-CAR (empty circles, n = 8 cells) or 3 µM C10-acyl-CAR (filled circles, n = 7 cells); Ab, typical example of C8-CAR (red) compared with PSS (black). Ba, effect of 3 µM C18-CAR (filled circles, n = 9 cells) on IhERG-V relationship. The inset shows the effect of 3 µM C18-CAR on end-pulse hERG current elicited by a depolarisation to −10 mV from a holding voltage of −70 mV, obtained on a representative cell (the arrows indicate the current obtained at −10 mV during an IV protocol, see Methods), ***, p<0.001. **, p<0.01.Bb, typical example of C18-CAR (red) compared with PSS (black).