Effect of ethanol on nef-mediated alteration of HCV replicon expression.

<p>RLuc cells were transfected with gfp- or nef.GFP-expressing plasmids, and the cells were treated with the indicated concentration of ethanol for 48 hr. Renillar luciferase activity in each transfected lysate was then measured. Means and standard deviation values of triplicate experiments are depicted as lines.</p>