Effect of TGFα on KGN cell viability and DNA synthesis.

<p><b>A</b>) KGN cells were treated with or without TGFα for 48 hours and cell viability was detected with MTT assay. Bars represent means ± SEM. Bars with different letters are significantly different (<i>P</i><0.05) from each other. <b>B</b>) Effect of TGFα on KGN cell DNA synthesis. KGN cells were treated with or without TGFα for 36 hours and DNA synthesis was detected by BrdU incorporation. A representative image for BrdU immunofluorescence is shown for both control and TGFα treated groups. Magnification, 200×. Bars represent means ± SEM. “a”, significantly different (<i>P</i><0.05) from the control group.</p>