Effect of STS on phosphorylation of AMPK in VSMCs.

(A) VSMCs were incubated with 10 µM STS for the indicated times in 5.5 mM glucose (NG). Protein expression of p-AMPK was determined by Western blot analysis. Results are representative of three independent experiments. (B) VSMCs were treated with the indicated concentration of STS for 24 h. Protein expression of p-AMPK was determined using Western blot analysis. Results are representative of three independent experiments. (C) VSMCs were treated with high concentrations of glucose (25 mM, HG) for 24 h in the presence of indicated concentrations of STS. Protein expression of p-AMPK was determined using Western blot analysis. Results are representative of three independent experiments.