Effect of SSHE on cell cycle and viability of pancreatic cancer cell lines.

(A) Cell cycle. Panc-1 cells were treated with vehicle alone, 100 μg/ml SSHE or 200 μg/ml SSHE for 20 h, fixed, stained with PI, and then subjected to cytometry. (B) Cell viability. Pancreatic cancer cell lines were treated with vehicle alone or various concentrations of SSHE for 42 h. Cell viability was assessed by the MTT assay. Bars; SD. (C) Effect of SSHE on normal cells. HUVEC, HPAEpiC and Panc-1 cells were treated with vehicle alone or 100 μg/ml SSHE for 38 h. Cell viability was assessed by the MTT assay. Bars; SD. (D) Morphology. Panc-1 cells were treated with vehicle alone (left) or 200 μg/ml SSHE for 40 h (right). (E) Effect of SSHE on Panc-1 cells under hypoxic conditions. Panc-1 cells were treated with vehicle alone or various concentrations of SSHE for 42 h. Cell viability was determined by the MTT assay.