Effect of LicA on the expression of uPA, as well as cell migration and inhibition after being treated with a JNK inhibitor (SP600125).
(A) SK-Hep-1 cells were pretreated with SP600125 (25 µM) for 2 h, and then incubated in the presence or absence of LicA (10 µM) for 24 h. Conditioned media was collected and uPA activity was measured using casein zymography. (B) uPA protein was determined by using western blotting. (C) Cells were fixed and immunostained with anti-uPA antibody (red), and cell nuclei were counterstained with DAPI reagent. (D) uPA mRNA expression levels were determined by RT-PCR. Cells were also assessed for migration (E) and invasion (F). Data are presented as the mean±SE of at least three independent experiments. *p<0.05, untreated cells versus SP600125 or LicA; # p<0.01, LicA versus SP600125 plus LicA.