Figure_5.tif (750.22 kB)
Effect of IN rVSV-EnvG4-G6 adminstration.
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posted on 2014-09-12, 02:51 authored by Svetlana Rabinovich, Rebecca L. R. Powell, Ross W. B. Lindsay, Maoli Yuan, Alexei Carpov, Aaron Wilson, Mary Lopez, John W. Coleman, Denise Wagner, Palka Sharma, Marina Kemelman, Kevin J. Wright, John P. Seabrook, Heather Arendt, Jennifer Martinez, Joanne DeStefano, Maria J. Chiuchiolo, Christopher L. Parks(a) Mean body weights and temperatures (b) of inoculated mice. (c) Mean copy numbers of VSV N genomic RNA or (d) VSV N mRNA per mg of indicated tissue or mL of blood. N = 4–12, dependent on study day. Fresh tissue specimens were homogenized, clarified by centrifugation and supernatants were subjected to RNA extraction and qPCR. All samples were tested in duplicate. Dotted lines indicate limits of detection. SEM is shown. *p<0.05 for comparison of rVSV-EnvG4-G6 to rVSV-G4. All PBS and rVSV-EnvG4-ΔG values were found to be significantly lower than rVSV-EnvG4-G6 and rVSV-G4 values.
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G positionSignificant quantitiesimmunogenic HIVConformationally Intacthumoral responsesnovel vectorcytoplasmic tail regionssurface glycoproteinG generegimen elicitingFunctional HIVflow cytometryEnvG functionalityIM VSVsurface EnvGEnv trimersHIV vaccine platformclade B Env immunogenattenuating quantitiesSIV challengestomatitis virusgrowth attenuationelisaattenuated HIVHIV immunogensElicits Potent CellularEnvelope TrimersWestern Blot analysissurface Gilrhesus macaquesneutralizing Ab titersIM electroporated plasmids encoding EnvGsafety concerns
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